Part:BBa_K314974:Design

CapDCP_F24Y_L40R_T59N

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 674
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Point mutation at residues 24, 40, 59 on Foldit design from Phenylalanine to Tyrosine, Leucine to Arginine, and Threonine to Asparagine, respectively. Foldit-created linker, Circular Permutation, No Signal Sequence, Single band on protein agarose gel

Source

In-House Mutant